The methods for the quantitative determination of technetium isotope ( 99Tc) in uranium materials based on inductively coupled plasma mass-spectrometry are described. The results of the investigation of uranium matrix effect on the analytical signal of 99Tc for two mass-spectrometer models are presented. Different approaches for the quantification of 99Tc in uranium materials are investigated including direct analysis and analysis with preliminary uranium separation. The methods for the quantification of 99Tc consisting of chromatographic separation of uranium and 99Tc using UTEVA and TEVA resins with consequent ICP-MS detection were developed. The detection limits of the developed methods are within the range of 0.0006 to 0.02 ng/g U. The choice of optimum conditions for quantitative determination of 99Tc by direct ICP-MS analysis allowed avoiding «tough» ICP-MS operating conditions which consist in high forward power equal to 1600 W. The developed methods require much less time and effort compared to radiometry methods. The introduction of the developed methods allow improving the existing standards for the quality control of the uranium materials in nuclear industry.