Voltammetric Determination of Measles Virus Antibodies Using a Glassy Carbon Electrode Modified with 2-Propargyltio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine

Standard

Voltammetric Determination of Measles Virus Antibodies Using a Glassy Carbon Electrode Modified with 2-Propargyltio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine. / Medvedeva, M.; Mazur, A.; Svalova, T. и др.
в: Journal of Analytical Chemistry, Том 78, № 12, 01.12.2023, стр. 1694-1700.

Результаты исследований: Вклад в журнал › Статья › Рецензирование

BibTeX

@article{4f18fb0498b24eeda53373b7782a00cc,

title = "Voltammetric Determination of Measles Virus Antibodies Using a Glassy Carbon Electrode Modified with 2-Propargyltio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine",

abstract = "A method is proposed for the voltammetric determination of measles antibodies using a glassy carbon electrode modified with 2-propargylthio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine. An analytical signal was generated using a synthesized original compound attached to the surface of carboxylated carbon nanotubes through a thioline reaction and acted as a cross-linker for the immobilization of a bioreceptor, the measles virus antigen. We selected optimal working conditions for determining measles antibodies: signal recording mode, incubation time, temperature, pH of measurements, and concentration of the immobilized antigen. A calibration dependence I* (%) = –(1.5 ± 0.7)log с(IU/mL) + (29 ± 2) (R2 = 0.9653) was linear in the concentration range of measles antibodies 0.1–5.0 IU/mL. The limit of detection equal to 0.17 IU/mL, calculated using the 3σ test, ensures the use of the proposed method for the rapid analysis of biological fluids.",

author = "M. Medvedeva and A. Mazur and T. Svalova and I. Balin and V. Rusinov and A. Matern and A. Kozitsina",

note = "This work was supported by the Russian Science Foundation, project no. 20-13-00142-P.",

year = "2023",

month = dec,

day = "1",

doi = "10.1134/S1061934823120109",

language = "English",

volume = "78",

pages = "1694--1700",

journal = "Journal of Analytical Chemistry",

issn = "1061-9348",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "12",

}

RIS

TY - JOUR

T1 - Voltammetric Determination of Measles Virus Antibodies Using a Glassy Carbon Electrode Modified with 2-Propargyltio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine

AU - Medvedeva, M.

AU - Mazur, A.

AU - Svalova, T.

AU - Balin, I.

AU - Rusinov, V.

AU - Matern, A.

AU - Kozitsina, A.

N1 - This work was supported by the Russian Science Foundation, project no. 20-13-00142-P.

PY - 2023/12/1

Y1 - 2023/12/1

N2 - A method is proposed for the voltammetric determination of measles antibodies using a glassy carbon electrode modified with 2-propargylthio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine. An analytical signal was generated using a synthesized original compound attached to the surface of carboxylated carbon nanotubes through a thioline reaction and acted as a cross-linker for the immobilization of a bioreceptor, the measles virus antigen. We selected optimal working conditions for determining measles antibodies: signal recording mode, incubation time, temperature, pH of measurements, and concentration of the immobilized antigen. A calibration dependence I* (%) = –(1.5 ± 0.7)log с(IU/mL) + (29 ± 2) (R2 = 0.9653) was linear in the concentration range of measles antibodies 0.1–5.0 IU/mL. The limit of detection equal to 0.17 IU/mL, calculated using the 3σ test, ensures the use of the proposed method for the rapid analysis of biological fluids.

AB - A method is proposed for the voltammetric determination of measles antibodies using a glassy carbon electrode modified with 2-propargylthio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine. An analytical signal was generated using a synthesized original compound attached to the surface of carboxylated carbon nanotubes through a thioline reaction and acted as a cross-linker for the immobilization of a bioreceptor, the measles virus antigen. We selected optimal working conditions for determining measles antibodies: signal recording mode, incubation time, temperature, pH of measurements, and concentration of the immobilized antigen. A calibration dependence I* (%) = –(1.5 ± 0.7)log с(IU/mL) + (29 ± 2) (R2 = 0.9653) was linear in the concentration range of measles antibodies 0.1–5.0 IU/mL. The limit of detection equal to 0.17 IU/mL, calculated using the 3σ test, ensures the use of the proposed method for the rapid analysis of biological fluids.

UR - http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85183665984

UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=001156728900008

U2 - 10.1134/S1061934823120109

DO - 10.1134/S1061934823120109

M3 - Article

VL - 78

SP - 1694

EP - 1700

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 12

ER -

ID: 52294948