Результаты исследований: Вклад в журнал › Статья › Рецензирование
Результаты исследований: Вклад в журнал › Статья › Рецензирование
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TY - JOUR
T1 - Study of the Fragmentation of the Antiviral Drug Triazavirin in a Collision Cell under Electrospray Ionization Conditions
AU - Klimova, T.
AU - Shevyrin, V.
AU - Ivanova, A.
AU - Kozitsina, A.
AU - Deev, S.
AU - Rusinov, V.
N1 - This work was carried out within the framework of the state assignment of the RF Ministry of Education and Science on topic “Diagnostics,” project no. 122021000030-1, with partial support from the Russian Foundation for Basic Research, project no. 20-38-90239.
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Azoloazines possess a wide spectrum of biological activity. For the analysis of new drugs of the azoloazine series, reliable methods of identification and quantitative analysis are necessary. Mass spectrometry is one of the main methods for the analysis of drugs and their metabolites in a biological matrix. Information about the main fragmentation pathways of these substances favors their reliable identification. In this study, fragmentation pathways of the protonated molecule of triazavirin, which is an antiviral agent from the azoloazine series, are studied. The experiments are carried out using high resolution tandem mass spectrometry with an electrospray ionization source. The directions of fragmentation of the compound in the collision cell are confirmed by pseudo-MS3 experiments, which are possible due to dissociation in the ion source, and by comparative data analysis of triazavirin analogs labeled with stable isotopes. It is found that, during dissociation in the ion source, two types of ions associated with the loss of the nitro group are formed, in contrast to fragmentation in the collision cell. The formation of basic product ions occurs due to the loss of substituents in the heterocyclic structure with the release of neutral molecules or radicals, and also as a result of reactions affecting the integrity of the triazolotriazine heterocyclic system. The information presented in this work may be also useful in the study of structurally similar compounds.
AB - Azoloazines possess a wide spectrum of biological activity. For the analysis of new drugs of the azoloazine series, reliable methods of identification and quantitative analysis are necessary. Mass spectrometry is one of the main methods for the analysis of drugs and their metabolites in a biological matrix. Information about the main fragmentation pathways of these substances favors their reliable identification. In this study, fragmentation pathways of the protonated molecule of triazavirin, which is an antiviral agent from the azoloazine series, are studied. The experiments are carried out using high resolution tandem mass spectrometry with an electrospray ionization source. The directions of fragmentation of the compound in the collision cell are confirmed by pseudo-MS3 experiments, which are possible due to dissociation in the ion source, and by comparative data analysis of triazavirin analogs labeled with stable isotopes. It is found that, during dissociation in the ion source, two types of ions associated with the loss of the nitro group are formed, in contrast to fragmentation in the collision cell. The formation of basic product ions occurs due to the loss of substituents in the heterocyclic structure with the release of neutral molecules or radicals, and also as a result of reactions affecting the integrity of the triazolotriazine heterocyclic system. The information presented in this work may be also useful in the study of structurally similar compounds.
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UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=001172871000008
U2 - 10.1134/S1061934823140034
DO - 10.1134/S1061934823140034
M3 - Article
VL - 78
SP - 1921
EP - 1928
JO - Journal of Analytical Chemistry
JF - Journal of Analytical Chemistry
SN - 1061-9348
IS - 14
ER -
ID: 53847359