Determination of toxic, genotoxic and cytotoxic effects of Drosophila melanogaster for Monarda fistulosa var. menthifolia, M. fistulosa, M. didyma and M. media was carried out. The Oregon-R line was used to test for toxic manifestations in a number of indicators: fecundity, early and late embryonic mortality of offspring and total lethality of individuals (LC50). The genotoxic effect was recorded using SMART. The change in the wing shape and regulated cell death was determined. The lethality of individuals grown on a nutrient medium with the addition of an extract of M. fistulosa var. menthifolia, as well as F1 offspring at the embryonic stage of development was studied. Genotoxicity was not registered for any of the tested extracts. The M. didyma extract is characterized by cytotoxic properties. An effect on viability was found for extracts of M. media and M. fistulosa var. menthifolia. Cytotoxic effect was observed when exposed to M. didyma extract. © 2023 Indian Drug Manufacturers' Association. All rights reserved.