TY - JOUR

T1 - Synthesis of Prussian Blue nanoparticles in water/alcohol mixtures

AU - Khramtsov, Pavel

AU - Kropaneva, Maria

AU - Kiselkov, Dmitriy

AU - Minin, Artem

AU - Chekanova, Larisa

AU - Rayev, Mikhail

N1 - This research was funded by the Russian Science Foundation, grant number 22–75–00025. Transmission electron microscopy investigations were performed in the Collaborative Access Center of the M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences. The authors thank the Core Facility of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, During the preparation of this work, the authors used OpenAI's tool, ChatGPT 3.5, to check grammar and rephrase certain sentences. The initial text was written in English by human authors. After applying ChatGPT, the authors diligently reviewed and edited the content as necessary, taking full responsibility for the publication's content. The authors thank Tatyana Bezukladnikova for English editing.

PY - 2024/4/1

Y1 - 2024/4/1

N2 - Prussian Blue, a blue coordination polymer has a very promising future in the realm of biomedicine. Its nanoparticles, known as catalytic labels or nanozymes. They exhibit remarkable peroxidase-like properties and serve as effective antioxidants. Demand for synthesizing Prussian Blue nanoparticles with customizable sizes is on the rise. This article unveil a novel approach to synthesizing Prussian Blue nanoparticles. A synthesis of Prussian Blue nanoparticles by reducing an equimolar mixture of FeCl3 and K3[Fe(CN)6] with hydrogen peroxide in different water-alcohol mixtures is demonstrated for the first time. Alcohols with a lower dielectric constant (propanol-1, isopropyl alcohol, and tert-butanol) contribute to an increase in nanoparticle size, particularly at mole fractions of 0.02–0.05 and beyond. Conversely, alcohols with a higher dielectric constant (ethanol, methanol, ethylene glycol, and propylene glycol, excluding glycerol) demonstrate the ability to decrease nanoparticle size at mole fractions of 0.2–0.26 and higher. A scalable and reproducible method for preparing 30–40 nm Prussian Blue nanoparticles using 79.2% ethylene glycol as a solvent is presented. The proposed mechanism behind the effect of ethylene glycol involves the limitation of both growth and secondary aggregation of Prussian Blue nanoparticles. These synthesized nanoparticles prove their efficiency as catalytic labels in a model vertical flow immunoassay designed to detect antibodies against SARS-CoV-2.

AB - Prussian Blue, a blue coordination polymer has a very promising future in the realm of biomedicine. Its nanoparticles, known as catalytic labels or nanozymes. They exhibit remarkable peroxidase-like properties and serve as effective antioxidants. Demand for synthesizing Prussian Blue nanoparticles with customizable sizes is on the rise. This article unveil a novel approach to synthesizing Prussian Blue nanoparticles. A synthesis of Prussian Blue nanoparticles by reducing an equimolar mixture of FeCl3 and K3[Fe(CN)6] with hydrogen peroxide in different water-alcohol mixtures is demonstrated for the first time. Alcohols with a lower dielectric constant (propanol-1, isopropyl alcohol, and tert-butanol) contribute to an increase in nanoparticle size, particularly at mole fractions of 0.02–0.05 and beyond. Conversely, alcohols with a higher dielectric constant (ethanol, methanol, ethylene glycol, and propylene glycol, excluding glycerol) demonstrate the ability to decrease nanoparticle size at mole fractions of 0.2–0.26 and higher. A scalable and reproducible method for preparing 30–40 nm Prussian Blue nanoparticles using 79.2% ethylene glycol as a solvent is presented. The proposed mechanism behind the effect of ethylene glycol involves the limitation of both growth and secondary aggregation of Prussian Blue nanoparticles. These synthesized nanoparticles prove their efficiency as catalytic labels in a model vertical flow immunoassay designed to detect antibodies against SARS-CoV-2.

UR - http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85185008170

UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=001187696100001

U2 - 10.1016/j.colsurfa.2024.133446

DO - 10.1016/j.colsurfa.2024.133446

M3 - Article

VL - 686

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - 133446

ER -