Research output: Contribution to journal › Article › peer-review
Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Novel electrochemical immunosensing platform based on magnetite-antibody conjugate as a direct signal label: design and application for Salmonella typhimurium antigen determination
AU - Svalova, Tatiana S.
AU - Malysheva, Natalia
AU - Zaidullina, Regina A.
AU - Medvedeva, Margarita
AU - Mazur, Alena V.
AU - Morshchinin , Ivan V.
AU - Kozitsina, Alisa N.
N1 - The research was supported by the Council for Grants of the President of the Russian Federation under Grant MK-392.2022.1.3 and the Ministry of Science and Higher Education of the Russian Federation (Ural Federal University project within the Priority-2030 Program.
PY - 2023/11/2
Y1 - 2023/11/2
N2 - A novel electrochemical sensing platform based on Fe3O4-antibody conjugate was developed for the rapid and sensitive determination of Salmonella typhimurium antigen in model suspensions. The conjugates Fe3O4-antibody were synthesized via carbodiimide cross-linking and characterized by infrared spectroscopy and cyclic voltammetry. The electrochemical properties of Fe3O4 magnetic nanoparticles in water were investigated; the possible mechanism of direct electrochemical reduction was proposed and justified as an analytical signal. The design of an electrochemical sensing platform with magnetically controlled flows has been proposed. Under the most appropriate conditions, the developed portable device was provided the linear response as I (µA) = (7.20 ± 0.35) × (–lg CAg) − (23.77 ± 1.95) in the concentration range 1 × 10−7–1 × 10−4mg/mL. The detection limit was 390 ng/mL and was comparable to other works.
AB - A novel electrochemical sensing platform based on Fe3O4-antibody conjugate was developed for the rapid and sensitive determination of Salmonella typhimurium antigen in model suspensions. The conjugates Fe3O4-antibody were synthesized via carbodiimide cross-linking and characterized by infrared spectroscopy and cyclic voltammetry. The electrochemical properties of Fe3O4 magnetic nanoparticles in water were investigated; the possible mechanism of direct electrochemical reduction was proposed and justified as an analytical signal. The design of an electrochemical sensing platform with magnetically controlled flows has been proposed. Under the most appropriate conditions, the developed portable device was provided the linear response as I (µA) = (7.20 ± 0.35) × (–lg CAg) − (23.77 ± 1.95) in the concentration range 1 × 10−7–1 × 10−4mg/mL. The detection limit was 390 ng/mL and was comparable to other works.
UR - http://www.scopus.com/inward/record.url?partnerID=8YFLogxK&scp=85148626793
UR - https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=tsmetrics&SrcApp=tsm_test&DestApp=WOS_CPL&DestLinkType=FullRecord&KeyUT=000935806200001
U2 - 10.1080/00032719.2023.2180015
DO - 10.1080/00032719.2023.2180015
M3 - Article
VL - 56
SP - 2572
EP - 2585
JO - Analytical Letters
JF - Analytical Letters
SN - 0003-2719
IS - 16
ER -
ID: 43266875