FIELD: electrochemical analysis. SUBSTANCE: method is disclosed for determining the content of chloramphenicol in the analysed aqueous medium using a carbon-containing working electrode modified with 3,6-bis[(trimethylsilyl)ethynyl]-9H-carbazole, including molecular recognition of the chloramphenicol molecule in an aqueous medium by this organic molecule by immersing the electrode in an electrochemical cell, containing 0.05% solution 3,6-bis[(trimethylsilyl)ethynyl]-9H-carbazole in acetonitrile, applying three cyclic potential scans in the range of 0.3 to 1.8 V to form an electrodeposited layer of 3,6-bis[(trimethylsilyl)ethynyl]-9H-carbazole on the electrode, moving the electrode into the aqueous test solution, keeping it in it for 30 minutes, then detecting a direct electrochemical signal from chloramphenicol by transferring the electrode into an aqueous solution of the background electrolyte 0.1 M KCl, recording a linear voltammogram in the range from 0 to -0.8 V and determining the chloramphenicol content by the electroreduction current of the nitro group in the chloramphenicol structure using the method calibration chart. EFFECT: selectivity and sensitivity of the analysis with implementation of a small number of stages. 1 cl, 2 dwg, 2 ex.