The application of the ecosystem approach to enterprise development can be applied both at the global level and at the level of individual regions. In the framework of the study, the author identified the regions of the Russian Federation that have significant results on the world stage of activity of actively growing small business companies, the so-called "startup". And the documentary analysis of the experience of business development in these regions of the country was conducted. On the basis of the obtained data, proposals for improving business activities on the basis of the ecosystem approach are formed.