The development of the world economy, in general, and of the domestic one, in particular, forces the regional enterprises to think more of transition to the innovative way of development, where knowledge acts as the main resource. First of all, it is the knowledge-intensive and hi-tech productions. The innovative activity of such enterprises allows regions to remain competitive and forces them to tap into new markets, to increase the quality of education for providing the level of scientific personnel, to develop new public, social and financial organizations. During the research, we have analyzed relevant approaches to the improvement of the knowledge generation system through the management of life cycle, put forward the conclusion about the need for the elaboration of the single approach for the hi-tech enterprises of the region; revealed the new models of the generation of knowledge for the regional hi-tech enterprises, however, their imperfection help carrying out additional research.