FIELD: medicine.
SUBSTANCE: invention refers to biotechnology, particularly to a microorganism test in various objects and media. The method provides conjugating electrically Fe0, MgFe2O4 or Fe3O4 labelled bacteria in an aqueous medium at specified parameters. Unbounded nanoparticles are separated with using a magnetic field, and a working electrode made of gold, platinum or graphite-bearing materials and having a surface pre-modified by antibodies specific to a bacterial strain to be tested is immersed into an analysed solution. The electrode is kept at the specified parameters to form an immune complex on its surface and washed in a buffer solution containing normal horse serum and Tween-20. The electrode is removed from the solution and placed into a cell containing LiClO4, dissolved in acetonitrile, dimethyl formamide or dimethyl sulphoxide; the bacterial count is determined by a value of analytical oxidation of the nanoparticles localised in the immune complex on the surface of the working electrode.
EFFECT: invention enables providing higher analysis sensitivity, better output and simplified analysis procedure.
Translated title of the contributionELECTROCHEMICAL IMMUNOASSAY METHOD FOR MICROORGANISM TEST: patent of invention
Original languageRussian
Patent number2538153
IPCC12N 1/02,C12Q 1/04,G01N 33/53,B82B 1/00
Filing date22/03/2013
Publication statusPublished - 10 Jan 2015

ID: 11066626